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Writing bootloader with PICkit 3

Run MPLABX installer

Sellect

Run MPLAB IPE

Select device and tool

Family : Advanced 8-bit MCUs (PIC18)
Device : PIC18F45K50
Tool : PICkit3

Connect power to your USB-IOBOARD using microUSB cable.
Then, connect PICkit3.

Apply && Connect

Bootloader Download

Select the bootloader hex file.

Run Program